최근 급격한 발전을 이룬 공간 전사체 기법은 조직을 이루는 세포에 대한 매우 광범위한 정보를 제공해 준다. 공간 멀티오믹스 연구실에서는 기존의 기술로는 검출이 어렵거나 불가능한 다양한 생물학적 대상을 차세대 염기분석기 혹은 형광 이미징 기법을 이용하여 측정하는 방법을 새로이 개발하는 연구를 수행한다. 특히 새로운 단일 세포 및 공간 전사체 혹은 다양한 멀티오믹스의 분석법을 개발하여 임상 조직에서 발견되는 세포 및 분자적 이형성 및 병리적 세포구조를 발견, 이해하려고 한다. 또한 새로운 조직 팽창기법을 이용한 초고해상도 이미징을 통해 시냅스 단백질이 이루는 나노클러스터 구조를 이해하고자 하며 항체 접합물, AI-기반 항체/단백질 설계를 통해 차세대 공간 멀티오믹스 기술을 개발하고자 한다.

Recent rapid advancements in spatial transcriptomics techniques have provided extensive information about the cyto-architecture of tissue. The Spatial Multi-omics Laboratory conducts research to develop new methods for measuring various biomolecules that are difficult or impossible to detect with existing technologies, using next-generation sequencing or fluorescence imaging techniques. In particular, we aim to develop new single-cell and spatial transcriptomics or various multi-omics analysis methods to discover and understand cellular and molecular heterogeneity and pathological cell structures found in clinical tissues. Additionally, we seek to understand the nanocluster structure formed by synaptic proteins through super-resolution imaging using new tissue expansion techniques, and to develop next-generation spatial multi-omics technologies through antibody conjugates and AI-based antibody/protein design.

• Han-Eol Park†,Yu Tak Lee†, Junsuk Lee, Hyelin Ji, Young-Lan Song, Jae Won Lee, Sung-Yon Kim, Junho K. Hur, Eunha Kim, Chung Whan Lee, Yoon Dae Han, Hyunki Kim, and Chang Ho Sohn*, Fixative eXchange (FX)-seq: scalable single-nucleus RNA sequencing enables transcriptome profiling of heavily PFA-fixed or FFPE mouse brain and human cancer tissue, under review.
• Kang-Hoon Kim†, Jubeen Yoon†, Christian P. Macks†, Han-Eol Park, Jihyun Youn, Jung-uk Lee, Minji An, Jongseong Park, Jaewon Ko, and Chang Ho Sohn*, Molecular decrowding by tissue expansion allows precise determination of the spatial distribution of synaptic proteins at a nanometer scale by exTEM, ACS Nano, 2023, 17, 9919-9937.
• Han-Eol Park, Song Hyun Jo†, Rosalind H. Lee†, Christian P. Macks, Taeyun Ku, Jihwan Park, Chung Whan Lee, Junho K. Hur, and Chang Ho Sohn*, Spatial transcriptomics: technical aspects of recent developments and their applications in neuroscience and cancer research, Adv. Sci., 2023, 10, 2206939.
• Young-Gyun Park†, Chang Ho Sohn†, Ritchie Chen†, Margaret McCue, Dae Hee Yun, Gabrielle T. Drummond, Taeyun Ku, Nicholas B. Evans, Hayeon Caitlyn Oak, Wendy Trieu, Heejin Choi, Xin Jin, Varoth Lilascharoen, Ji Wang, Matthias C. Truttmann, Helena W. Qi, Hidde L. Ploegh, Todd R. Golub, Shih-Chi Chen, Matthew P. Frosch, Heather J. Kulik, Byung Kook Lim, Kwanghun Chung*, Protection of tissue physicochemical properties using polyfunctional crosslinkers, Nat. Biotechnol., 2019, 37, 73-83.